A Handbook of Mouse Models of Cardiovascular Disease
نویسندگان
چکیده
The gentle and careful use of laboratory animals requires specific knowledge on thegeneral biology and the characteristic hallmarks of the used animals and the adequateconsideration of legal requirements, ethical aspects, and scientific guidelines. In thischapter, important topics of laboratory animal science regarding the mouse as laboratoryanimal are addressed. Initially, a brief historical overview of the mouse is given and thestep from the house mouse to the worldwide actually mostly used laboratory animalspecies. Moreover, housing conditions of mice, maintaining, and selected geneticprinciples are described. Commonly used handling techniques are explained in detail.For practical benefit, adequate methods for the sampling of blood and bone marrowcells and the application of substances, in particular, the intravenous injection technique,are described. Finally, useful techniques for anesthesia, analgesia, and euthanasia areemphasized.
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